Journal: International Journal of Molecular Sciences

Article Title: APP Knock-In Mice Produce E22P-Aβ Exhibiting an Alzheimer’s Disease-like Phenotype with Dysregulation of Hypoxia-Inducible Factor Expression

doi: 10.3390/ijms232113259

Figure Lengend Snippet: Alzheimer’s disease (AD)-related histological changes in the brain of APP NL-P-F/NL-P-F mice. ( A ) The Aβ plaque deposition was detected by 82E1 in the hippocampal CA1, DG, and cortex of APP NL-P-F/NL-P-F mice from three to twelve months. The scale bar indicates 1 mm in representative coronal sections and 100 μm in each region). ( B ) The Aβ plaque area in the hippocampal CA1, DG, and the cortex of APP NL-P-F/NL-P-F mice from three to twelve months. WT ( n = 6) and NLPF ( n = 7–9). ( C , D ) Representative ( C ) fluorescent and ( D ) confocal images of tau phosphorylation were detected by PHF-1 (Ser396/Ser404) in the hippocampal CA1, CA3, and DG after twelve months. The scale bar indicates ( C ) 1 mm (50 μm in enlarged images) and ( D ) 50 μm (25 μm in enlarged images of CA1 area). ( E ) After twelve months, the phosphorylated tau-positive area (% of total area) in the hippocampal CA1, CA3, and DG. WT and NLPF ( n = 6). ( F , G ) After twelve months, representative ( F ) fluorescent and ( G ) confocal images of NeuN in the hippocampal CA1 and CA3. The scale bar indicates ( F ) 250 μm (50 μm in enlarged images) and ( G ) 50 μm. ( H ) After twelve months, the number of NeuN-positive neurons in the hippocampal CA1 and CA3. WT ( n = 17 slices/6 mice), NLPF ( n = 13 slices/6 mice). The values indicate the mean ± SEM. * p < 0.05, ** p < 0.01, *** p < 0.001, compared with age-matched WT.

Article Snippet: Mouse Anti-Human Amyloid β antibody (82E1) , 1:1000 , Immuno-Biological Laboratories, Gunma, Japan , 10323.

Techniques:

Journal: International Journal of Molecular Sciences

Article Title: APP Knock-In Mice Produce E22P-Aβ Exhibiting an Alzheimer’s Disease-like Phenotype with Dysregulation of Hypoxia-Inducible Factor Expression

doi: 10.3390/ijms232113259

Figure Lengend Snippet: Primary antibodies used for immunohistochemistry (IHC).

Article Snippet: Mouse Anti-Human Amyloid β antibody (82E1) , 1:1000 , Immuno-Biological Laboratories, Gunma, Japan , 10323.

Techniques: Immunohistochemistry

Journal: Frontiers in Cellular Neuroscience

Article Title: Alzheimer’s disease like neuropathology in Down syndrome cortical organoids

doi: 10.3389/fncel.2022.1050432

Figure Lengend Snippet: List of reagents and chemicals.

Article Snippet: Amyloid beta (N) (82E1) anti-human mouse IGG , 10323 , IBL-America.

Techniques: Staining, Protease Inhibitor, Imaging, Enzyme-linked Immunosorbent Assay

Journal: Frontiers in Cellular Neuroscience

Article Title: Alzheimer’s disease like neuropathology in Down syndrome cortical organoids

doi: 10.3389/fncel.2022.1050432

Figure Lengend Snippet: Abnormal Aß accumulation in DS iPSCs-derived cortical organoids. (A) A representative Aß immunostaining in 12 weeks DS and isogenic control organoids with two different antibodies D54D2 (Aß37-42, green) and 82E1 (Aß40-42, red). (B) Immunohistochemical analysis of Aß antibodies D54D2 and 82E1 revealed a significantly increased Aß immunoreactivity in DS organoids ( n = 10–11, p < 0.01). (C) A representative Aß plaque staining in DS and control organoids using Amylo-Glo. (D) Immunohistochemical analysis of Amylo-Glo revealed a significantly increased amyloid plaque load in DS organoids ( n = 8, p < 0.05). (E) Aß40 and Aß42 in the soluble and insoluble fractions of 8 weeks and 12 weeks DS and control organoids were quantified using Elisa and the ratio of Aß42/Aß40 was significantly increased in the insoluble fractions of 12 weeks DS organoids ( n = 10–11, p < 0.05). * p < 0.05, and ** p < 0.01 vs. Control.

Article Snippet: Amyloid beta (N) (82E1) anti-human mouse IGG , 10323 , IBL-America.

Techniques: Derivative Assay, Immunostaining, Control, Immunohistochemical staining, Staining, Enzyme-linked Immunosorbent Assay